Structure, assembly and function of the surface envelope (fertilization envelope) from eggs of the sea urchin, Strongylocentrotus purpuratus.
作者： Edward J. Carroll , Mildred Acevedo-Duncan , Robin Wilby Justice , Lyric Santiago
DOI: 10.1007/978-1-4613-2255-9_16
关键词: Strongylocentrotus purpuratus 、 Biophysics 、 Fertilization envelope 、 Cortical granule 、 Divalent 、 Protease 、 Chemistry 、 Kunitz STI protease inhibitor 、 Vitelline membrane 、 Sea urchin
摘要: The sea urchin fertilization envelope (FE) is formed following initial sperm-egg interaction from the egg surface vitelline (VE) and paracrystalline protein fraction (PCF), derived cortical granules. Although mature FEs are physicochemically hardened postinsemination, a major consisting of seven polypeptides was extracted Strongylocentrotus purpuratus major, separated components were immunologically cross-reactive with principal in PCF isolated Antibodies prepared against extracted, core crossreactive VEs, but not PCF, suggesting that only VE covalently crosslinked. Based on protease inhibitor experiments, our model FE development benzamidine-sensitive, granule cleaves 200 kD polypeptide during elevation to set up morphological change papillae which occurs later. Divalent cations precipitate form metal proteinate bridges between PCF. peroxidase we suggest crosslinks polypeptides, beginning at 2-3 min restrict permeability so normal thickening occurs. A 305 appears be important based inhibition sperm binding by antibodies purified polypeptide.
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