Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum
作者： Laura Liscum , Kenneth L. Luskey , Sandip K. Basu , Hiroto Okayama , Paul Berg
DOI: 10.1038/308613A0
关键词: 7-Dehydrocholesterol reductase 、 Protein primary structure 、 Coenzyme A 、 Transmembrane protein 、 Molecular biology 、 Reductase 、 Signal peptide 、 Biology 、 Nucleic acid sequence 、 Biochemistry 、 Endoplasmic reticulum
摘要: The nucleotide sequence of a 4.8-kilobase mRNA for hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase, the endoplasmic reticulum enzyme that controls cholesterol biosynthesis, shows it is protein 887 amino acids (molecular weight 97,092) which contains three potential sites asparagine-linked glycosylation. reductase transmembrane glycoprotein, but in contrast to many other glycoproteins, lacks cleavable or Hydrophobie NH2-terminal signal sequence.
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utsystem.edu参考文章(34)
Yoshiie OKADA, Alan B. FREY, Thomas M. GUENTHNER, Franz OESCH, David D. SABATINI, Gert KREIBICH, Studies on the biosynthesis of microsomal membrane proteins. Site of synthesis and mode of insertion of cytochrome b5, cytochrome b5 reductase, cytochrome P-450 reductase and epoxide hydrolase. FEBS Journal. ,vol. 122, pp. 393- 402 ,(1982) , 10.1111/J.1432-1033.1982.TB05894.X
K L Luskey, J R Faust, D J Chin, M S Brown, J L Goldstein, Amplification of the gene for 3-hydroxy-3-methylglutaryl coenzyme A reductase, but not for the 53-kDa protein, in UT-1 cells. Journal of Biological Chemistry. ,vol. 258, pp. 8462- 8469 ,(1983) , 10.1016/S0021-9258(20)82087-0
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
Ranajit Roychoudhury, Ray Wu, [7] Terminal transferase-catalyzed addition of nucleotides to the 3′ termini of DNA Methods in Enzymology. ,vol. 65, pp. 43- 62 ,(1980) , 10.1016/S0076-6879(80)65009-5
L Liscum, K L Luskey, D J Chin, Y K Ho, J L Goldstein, M S Brown, Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. Journal of Biological Chemistry. ,vol. 258, pp. 8450- 8455 ,(1983) , 10.1016/S0021-9258(20)82085-7
Gert Kreibich, Eugene E. Marcantonio, David D. Sabatini, Ribophorins I and II: membrane proteins characteristic of the rough endoplasmic reticulum. Methods in Enzymology. ,vol. 96, pp. 520- 530 ,(1983) , 10.1016/S0076-6879(83)96045-7
M S Brown, J L Goldstein, Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth Journal of Lipid Research. ,vol. 21, pp. 505- 517 ,(1980) , 10.1016/S0022-2275(20)42221-7
H Okayama, P Berg, A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Molecular and Cellular Biology. ,vol. 3, pp. 280- 289 ,(1983) , 10.1128/MCB.3.2.280
William A. Braell, Harvey F. Lodish, The erythrocyte anion transport protein is cotranslationally inserted into microsomes Cell. ,vol. 28, pp. 23- 31 ,(1982) , 10.1016/0092-8674(82)90371-3
L. Liscum, R. D. Cummings, R. G. Anderson, G. N. DeMartino, J. L. Goldstein, M. S. Brown, 3-Hydroxy-3-methylglutaryl-CoA reductase: a transmembrane glycoprotein of the endoplasmic reticulum with N-linked "high-mannose" oligosaccharides. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 7165- 7169 ,(1983) , 10.1073/PNAS.80.23.7165