Role of Nitrogenase and Ferredoxin in the Mechanism of Bioelectrocatalytic Nitrogen Fixation by the Cyanobacteria Anabaena variabilis SA-1 Mutant Immobilized on Indium Tin Oxide (ITO) Electrodes

摘要: Abstract Current ammonia production methods are costly and environmentally detrimental. Biological nitrogen fixation has implications for low cost, friendly production. It been shown that electrochemical stimulation increases the output of cyanobacteria SA-1 mutant Anabaena variabilis , but mechanism bioelectrocatalysis unknown. Here, electrostimulated biological is investigated by immobilization with polyvinylamine on indium tin oxide (ITO) coated polyethylene. Cyclic voltammetry performed in absence presence various substrates nitrogenase repressed derepressed cells to study mechanism, cyclic UV–vis spectroscopy used identify redox moieties spent electrolyte. A bioelectrocatalytic signal observed A. N 2 light. Results indicate protein ferredoxin mediates electron transfer between electrode stimulate