Primary and recall cytotoxic T lymphocyte responses to autologous antigen in HIV-1-infected subjects

摘要: Human immunodeficiency virus type-1 (HIV-1) affects more than 30 million people worldwide and has accounted for over deaths. The advent of combination antiretroviral therapy (cART) resulted in a drastic decrease AIDS-associated morbidity mortality. Despite this, cART fails to completely eradicate the from infected patients, as cessation treatment results rebound viremia resumption disease progression. Partial immune reconstitution is achieved under suppressive therapy, thus causing research efforts begin development curative strategies HIV-1-infected patients. We believe best method HIV-1 eradication will be through cytotoxic T lymphocyte (CTL) elimination latently-infected reservoir, which may difficult given propensity undergo mutations that evade CTL recognition. Dendritic cells, most potent antigen-presenting can reveal broad robust HIV-1-specific cell responses subjects on prime CD8+ cells specific various antigens. therefore inducing anti-HIV-1 response use DC-based immunotherapy targeting patient’s own, unique (autologous) virus. It unclear, however, if naive repertoire function been sufficiently restored respond autologous containing multiple mutational variants. In present study, we longitudinally evaluated evolution changes throughout untreated treated infection Multicenter AIDS Cohort Study (MACS). show dendritic antigen-specific at all stages progression are inducers polyfunctional after long-term cART. developed utilized an vitro model DC memory subsets first time primed target latent reservoir. Taken together, these findings shed new light viral variants support