Nitrite Uptake into Intact Pea Chloroplasts II. Influence of Electron Transport Regulators, Uncouplers, ATPase and Anion Uptake Inhibitors and Protein Binding Reagents

摘要: The relationship between net nitrite uptake and its reduction in intact pea chloroplasts was investigated employing electron transport regulators, uncouplers, photophosphorylation inhibitors. Observations confirmed the dependence of on stromal pH but also suggested a partial dependance upon PSI phosphorylation. It that ammonia stimulates nitrogen assimilation dark by association with protons. Inhibition N-ethylmaleimide dinitrofluorobenzene could not be completely attributed to their inhibition carbon dioxide fixation. Other protein binding reagents which inhibited photosynthesis showed no effect uptake, except for p-chlormercuribenzoate stimulated uptake. results tended support proposed presence permeation channel addition HNO2 penetration. On basis lack known anion inhibitors, it concluded mechanism distinct from phosphate chloride/sulfate transport.