Reactive oxygen species (ROS) control the expression of Bcl-2 family proteins by regulating their phosphorylation and ubiquitination.
作者： Dechao Li , Eisaku Ueta , Tsuyoshi Kimura , Tetsuya Yamamoto , Tokio Osaki
DOI: 10.1111/J.1349-7006.2004.TB03323.X
关键词: Pyrrolidine dithiocarbamate 、 Intracellular 、 Protein kinase A 、 Bcl-2 family 、 Apoptosis 、 Biology 、 Ubiquitin 、 Phosphorylation 、 Reactive oxygen species 、 Molecular biology
摘要: We examined the influence of ROS on phosphorylation and complex formation Bcl-2 family proteins in Mn-superoxide dis-mutase (SOD) antisense-transfected squamous cell carcinoma cells, OSC-4 cells. The increase intracellular level induced by cis-diamminedichloroplatinum (CDDP) γ-ray treatment was greater cells than control vector-trans-fected apoptosis more extensively former. Antisense-transfected expressed high levels Bax Bak, but low Bcl-XL when treated with CDDP, peplomycin, 5-fluorouracil or γ-rays. After these agents, protein kinase A, (Thr56) Bad (Ser155) increased, especially antioxidant (N-acetylcysteine pyrrolidine dithiocarbamate)-pretreated were very ubiquitination decreased although their increased antioxidants. These results reveal that induce regulating proteins, resulting proapoptotic antiapoptotic expression.
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