Release of ATP induced by hypertonic solutions in Xenopus oocytes

摘要: ATP mediates intercellular communication. Mechanical stress and changes in cell volume induce release from various types, both secretory non-secretory. In the present study, we stressed Xenopus oocytes with a hypertonic solution enriched mannitol (300 mm). We measured simultaneously ionic currents single oocyte. A decrease volume, activation of an inward current were coincident. found two components release: first was associated granule or vesicle exocytosis, because it inhibited by tetanus neurotoxin, second related to current. exponential described correlation between hypertonic-activated Gadolinium ions, which block mechanically activated channels, but did not affect volume. Oocytes expressing CFTR (cystic fibrosis transmembrane regulator) released under shock, significantly component: that exocytosis. Since is balance degradation ecto-enzymes, nucleoside triphosphate diphosphohydrolase (NTPDase) activity oocyte surface during osmotic stress, as calcium-dependent hydrolysis ATP, more than 50 % conditions. The best-characterized membrane protein showing NTPDase CD39. injected antisense oligonucleotide complementary CD39 mRNA less showed lower amplitude those water.