The MobA-Linked Primase Is the Only Replication Protein of R1162 Required for Conjugal Mobilization

作者: Dorian Henderson , Richard Meyer

DOI: 10.1128/JB.181.9.2973-2978.1999

关键词: Origin recognition complexPlasmidBiologyPre-replication complexDNA replicationReplication factor CControl of chromosome duplicationGeneticsPrimasePlasmid preparation

摘要: Cells newly transformed with plasmid R1162 DNA were used as donors in conjugal matings to determine if the replication genes are necessary for transfer. An intact system vegetative is not required transfer at normal frequency, but primase, form linked nickase, must be present donor cells.

参考文章(38)
R Meyer, M Hinds, M Brasch, Properties of R1162, a broad-host-range, high-copy-number plasmid. Journal of Bacteriology. ,vol. 150, pp. 552- 562 ,(1982) , 10.1128/JB.150.2.552-562.1982
E C Becker, H Zhou, R J Meyer, Replication of a plasmid lacking the normal site for initiation of one strand. Journal of Bacteriology. ,vol. 178, pp. 4870- 4876 ,(1996) , 10.1128/JB.178.16.4870-4876.1996
M Bhattacharjee, X M Rao, R J Meyer, Role of the origin of transfer in termination of strand transfer during bacterial conjugation. Journal of Bacteriology. ,vol. 174, pp. 6659- 6665 ,(1992) , 10.1128/JB.174.20.6659-6665.1992
M A Brasch, R J Meyer, Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. Journal of Bacteriology. ,vol. 167, pp. 703- 710 ,(1986) , 10.1128/JB.167.2.703-710.1986
L Y Li, N B Shoemaker, G R Wang, S P Cole, M K Hashimoto, J Wang, A A Salyers, The mobilization regions of two integrated Bacteroides elements, NBU1 and NBU2, have only a single mobilization protein and may be on a cassette. Journal of Bacteriology. ,vol. 177, pp. 3940- 3945 ,(1995) , 10.1128/JB.177.14.3940-3945.1995