Mitotic checkpoint gene expression is tuned by coding sequences

摘要: The mitotic checkpoint (also called spindle assembly checkpoint, SAC) is a signaling pathway that safeguards proper chromosome segregation. Proper functioning of the SAC depends on adequate protein concentrations and appropriate stoichiometries between proteins. Yet very little known about gene expression. Here, we show in fission yeast (S. pombe) combination short mRNA half-lives long supports stable levels. For genes mad2+ mad3+, their depend high frequency non-optimal codons destabilization mediated through RNA helicase Ste13 (S.c. Dhh1). In contrast, mad1+ half-life despite relatively optimal lack destabilizing motifs its mRNA. Hence, although they are functionally related, different employ strategies Taken together, propose codon usage fine-tuned for function. Our work shines light expression features promote function suggests synonymous mutations may weaken checkpoint.