Intermittent Hypoxia-Induced Carotid Body Chemosensory Potentiation and Hypertension Are Critically Dependent on Peroxynitrite Formation

摘要: Oxidative stress is involved in the development of carotid body (CB) chemosensory potentiation and systemic hypertension induced by chronic intermittent hypoxia (CIH), main feature obstructive sleep apnea. We tested whether peroxynitrite (ONOO−), a highly reactive nitrogen species, enhanced CB oxygen chemosensitivity during CIH. Accordingly, we studied effects Ebselen, an ONOO− scavenger, on 3-nitrotyrosine immunoreactivity (3-NT-ir) CB, discharge, arterial blood pressure (BP) rats exposed to Male Sprague-Dawley were CIH (5% O2, 12 times/h, 8 h/day) for 7 days. Ebselen (10 mg/kg/day) was administrated using osmotic minipumps BP measured with radiotelemetry. Compared sham animals, CIH-treated showed increased 3-NT-ir within responses hypoxia, response acute hypertension. Rats treated displayed significant reduction levels (60.8 ± 14.9 versus 22.9 4.2 a.u.), reduced 5% O2 (266.5 13.4 168.6 16.8 Hz), decreased mean (116.9 13.2 82.1 5.1 mmHg). Our results suggest that CIH-induced are critically dependent formation.