Analysis of nucleic acids by on-line liquid chromatography-Mass spectrometry

摘要: The numerous problems posed by modern biochemistry, biology, and medicine, as well the growing significance of genetic engineering require application fast reliable methods utmost sensitivity selectivity for analysis nucleic acids. High-performance liquid chromatography (HPLC) mass spectrometry (MS) represent established analytical techniques characterization structural elucidation single- double-stranded acids, ranging in size from a few nucleotides to several thousand base pairs. Although both are independently applicable acid analysis, on-line hyphenation significantly enhances their potential robust fully automable routine minute amounts biological samples. Among various chromatographic spectrometric modes available principle, ion-pair reversed-phase HPLC electrospray ionization (ESI-MS) have been shown be most suitable direct interfacing (LC) MS. Instrumental setup, experimental conditions, need carefully selected order maximize performance hyphenated system. Applications HPLC-ESI-MS include oligodeoxynucleotides synthesized solid-phase synthesis, antisense oligodeoxynucleotides, oligonucleotide metabolites, DNA adducts, genomic segments specifically amplified polymerase chain reaction (PCR), ribonucleic sizing restriction fragments, genotyping short tandem repeats (STRs) single nucleotide polymorphisms (SNPs), detection mutations sequences, sequencing