The demonstration of acid hydrolase, thermostable reduced diphosphopyridine nucleotide tetrazolium reductase and peroxidase activities in human lipofuscin pigment granules.

摘要: The characterization of human lipofuscin pigment granules as lysosomes is strengthened by the cytochemical demonstration β-glucuronidase and glucosaminidase activities in hepatocyte which also have acid phosphatase activity. Lysosomes parenchymal cells macrophages are seen to differ their content cytochemically demonstrable hydrolase activities. Reduced diphosphopyridine nucleotide tetrazolium reductase peroxidase that remarkably resistant heat (90°C for 15 min) prolonged fixation visualized granules. It suggested these oxidative reflect presence heme compounds accumulate during degradation cytoplasmic constituents autophagic vacuoles or dense bodies. metabolites with activity within may be responsible oxidation melanin precursors unsaturated fat alcohol-insoluble lipid pigment. Cytoplasmic iron (hemos...