Engineering Broader Specificity into an Antibiotic-Producing Polyketide Synthase
作者： Andrew F. A. Marsden , Barrie Wilkinson , Jesús Cortés , Nicholas J. Dunster , James Staunton
DOI: 10.1126/SCIENCE.279.5348.199
关键词: Biochemistry 、 Avermectin 、 Pseudonocardiaceae 、 Enzyme 、 Bacteria 、 Hybrid enzyme 、 Polyketide synthase 、 Saccharopolyspora erythraea 、 Biology 、 Antibiotics 、 Multidisciplinary
摘要: The wide-specificity loading module for the avermectin-producing polyketide synthase was grafted onto first multienzyme component (DEBS1) of erythromycin-producing in place normal module. Expression this hybrid enzyme erythromycin producer Saccharopolyspora erythraea produced several novel antibiotic erythromycins derived from endogenous branched-chain acid starter units typical natural avermectins. Because avermectin is known to accept more than 40 alternative carboxylic acids as units, approach opens way facile production analogs macrolides.
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