Haemolysis and turbidity influence on three analysis methods of quantitative determination of total and conjugated bilirubin on ADVIA 1650

摘要: Plasma bilirubin testing is crucial to prevent the occurrence of neonatal kernicterus. Haemolysis may occur during sampling and interfere with determination. Moreover, lipidic infusions induce plasma lipemia also measurement. We evaluated interference haemolysis three methods total direct measurement adaptated on an Advia 1650 analyser (Siemens Medical Solutions Diagnostics) : Synermed (Sofibel), Bilirubin 2 (Siemens) Auto FS (Diasys). The was little affected by all methods. method less sensitive even at low levels. conjugated significantly altered heamoglobin concentrations for FS(R) (30 microM or 0,192 g/100 mL haemoglobin) (60 0,484 haemoglobin). In marked contrast, we found no haemoglobin Direct reagent which complied validation criteria from French Society Biological Chemistry. up g/L Ivelip did not affect neither nor Diasys Siemens reagents. However, observed a strong starting 0,5 reagent. Our data suggest that both allow measure accurately in hemolytic lipemic samples, nevertheless, methodology these interferences.