Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins
作者： N.K. Stock , C. Escadafal , K. Achazi , M. Cissé , M. Niedrig
DOI: 10.1016/J.JVIROMET.2015.06.006
关键词: Yellow fever 、 Dengue virus 、 Immunofluorescence 、 Virus 、 Polyclonal antibodies 、 Virology 、 Biology 、 Japanese encephalitis 、 Capsid 、 Antiserum
摘要: There is still a considerable need for development of new tools and methods detecting specific viral proteins the diagnosis pathogenesis study Yellow fever virus (YFV). This aimed to develop characterize polyclonal peptide antisera detection YFV-C -NS1 proteins. The were used further investigate NS1 protein expression during YFV infection in mammalian cells. target detected by all western blot immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile Tick-borne encephalitis Japanese virus. Nuclear localization demonstrated first time. Experiments investigating suggested potential use YFV-NS1 diagnostic approaches targeting secreted form protein. described this offer possibilities research novel tests.
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