Electric fields and MAP kinase signaling can regulate early wound healing in lens epithelium.

摘要: METHODS. Scratch wounds were made in cultured bovine lens epithelial monolayers, and the exposed to an EF, with or without U0126 treatment (an inhibitor of active extracellular signal-regulated kinase [ERK 1/2]). Serial wound images taken areas measured. Western blot analysis immunocytochemical staining for ERK 1/2 wounded monolayers performed. RESULTS. An applied EF a given polarity influenced healing monolayer wounds. Wounds facing anode healed at normal rates, those cathode closed much more slowly. U0126, mitogenactivated protein (MAPK) signaling, inhibited healing, exposure EF. showed that both wounding application enhanced activation independently completely these activations monolayers. Immunocytochemical asymmetric EF-exposed wounds, weaker fluorescence cathode-facing which could contribute differentially directed wound-healing rates CONCLUSIONS. Exposure cathode. signaling pathways involved EF-directed migration edge. It may be possible use regulate aberrant cells results PCO after cataract surgery. (Invest Ophthalmol Vis Sci. 2003;44: 244 ‐249) DOI:10.1167/iovs.02-0456