Sphingomyelin Depletion from Plasma Membranes of Human Airway Epithelial Cells Completely Abrogates the Deleterious Actions of S. aureus Alpha-Toxin.
作者： Sabine Ziesemer , Nils Möller , Andreas Nitsch , Christian Müller , Achim Beule
关键词: Membrane 、 Cofilin 、 Cell biology 、 Actin depolymerizing factor 、 Hemolysin 、 Sphingomyelin 、 Transmembrane protein 、 Paracellular transport 、 Receptor 、 Chemistry
摘要: Interaction of Staphylococcus aureus alpha-toxin (hemolysin A, Hla) with eukaryotic cell membranes is mediated by proteinaceous receptors and certain lipid domains in host plasma membranes. Hla secreted as a 33 kDa monomer that forms heptameric transmembrane pores whose action compromises maintenance shape epithelial tightness. It not exactly known whether membrane cells facilitate adhesion Ha monomers, oligomerization, or pore formation. We used sphingomyelinase B, Hlb) expressed some strains staphylococci to pre-treat airway model order specifically decrease the sphingomyelin (SM) abundance their Such pre-incubation exclusively removed SM from fraction. abrogated formation heptamers prevented functional pores. exposure rHlb pre-treated did result increases [Ca2+]i, induce any microscopically visible changes paracellular gaps, hypo-phosphorylation actin depolymerizing factor cofilin usual. Removal human completely abrogates deleterious actions alpha-toxin.
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