Purification and Characterization of a Lectin from the Seeds of Amaranth (Amaranthus cruentus)
作者： S. J. KOEPPE , J. H. RUPNOW
DOI: 10.1111/J.1365-2621.1988.TB09289.X
关键词: Biochemistry 、 Sephadex 、 Affinity chromatography 、 Aspartic acid 、 Serine 、 Lectin 、 Amaranth 、 Fetuin 、 Amaranthus cruentus 、 Chromatography 、 Biology
摘要: A lectin (ACL) was purified from the seeds of Amaranthus cruentus using affinity chromatography on fetuin-agarose. Apparent homogeneity demonstrated by SDS-PAGE, Sephadex G100 and immunoprecipitation. The a dimer with an estimated molecular weight 66,000 subunit 35,000. ACL-mediated agglutination inhibited D-GalNAc fetuin. Thirty other carbohydrates were non-hibitory. ACL contained 2.2% neutral carbohydrate relatively high levels aspartic acid, glutamic serine glycine. heat labile retaining approximately 10% its original activity (titer) after 5 min at 70°C just 1 100°C.
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