Efficacy of ionic liquids on the growth and simultaneous xylanase production by Sporotrichum thermophile: membrane integrity, composition and morphological investigation
作者： Ayesha Sadaf , S. K. Khare
DOI: 10.1039/C6RA27979A
关键词: Thermophile 、 Xylanase 、 Membrane permeability 、 Catalysis 、 Chromatography 、 Hydrolysis 、 Ionic liquid 、 Enzyme 、 Membrane 、 Chemistry 、 Organic chemistry
摘要: Ionic liquids (ILs) are emerging as promising solvent systems for carrying out various biochemical reactions. The application of whole microbial cells catalysis in the presence ILs is gaining interest due to advantages being cost effective and providing a natural environment enzymes. Present study elucidates tolerance thermophilic fungus, S. thermophile, towards hydrophilic imidazolium based ILs. [EMIM][OAc], [BMIM][MeSO4] [BMIM][Otf] were found be compatible did not affect growth xylanase production whereas [HMIM][BF4] [BMIM][BF4] inhibited drastically. impact two types systems, [EMIM][OAc] non-compatible [BMIM][BF4], on thermophile membrane permeability, surface morphology, characteristics fatty acid composition studied by SEM, TEM, AFM FAME analysis. was tolerated upto 4.0% (v/v) concentrations cellular response this particular IL comparable with that control. In order explore feasibility using place its crude xylanase, conducted situ pre-treatment saccharification treated wheat straw high amount reducing sugars released compared controls. stable can thus utilised simultaneous saccharification.
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