Inducible Defense Mechanism against Nitric Oxide in Candida albicans
作者： Breanna D. Ullmann , Hadley Myers , Wiriya Chiranand , Anna L. Lazzell , Qiang Zhao
DOI: 10.1128/EC.3.3.715-723.2004
关键词: Nitric oxide 、 Biology 、 Immune system 、 Biochemistry 、 Microbiology 、 Candida albicans 、 Virulence 、 Yeast 、 Corpus albicans 、 Saccharomyces cerevisiae 、 Fungal protein
摘要: The yeast Candida albicans is an opportunistic pathogen that threatens patients with compromised immune systems. Immune cell defenses against C. are complex but typically involve the production of reactive oxygen species and nitrogen radicals such as nitric oxide (NO) damage or inhibit its growth. Whether defends itself NO molecules responsible for this defense have yet to be determined. in various bacteria Saccharomyces cerevisiae involves NO-scavenging flavohemoglobin. genome contains three genes encoding flavohemoglobin-related proteins, CaYHB1, CaYHB4, CaYHB5. To assess their roles metabolism, we constructed strains lacking each these demonstrated just one, consumption detoxification. In albicans, metabolic activity CaYHB1 mRNA levels rapidly induced by NO-generating agents. Loss increases sensitivity NO-mediated growth inhibition. mice, infections still resulted lethality, virulence was decreased compared wild-type strains. Thus, possesses a rapid, specific, highly inducible mechanism involving one putative flavohemoglobin genes.
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