The Spectrum of Mitochondrial DNA Deletions is a Ubiquitous Marker of Ultraviolet Radiation Exposure in Human Skin

摘要: We and colleagues have suggested that deletions of mitochondrial DNA may be useful as a biomarker ultraviolet radiation exposure in skin. In this study using southwestern approach involving monoclonal antibodies against thymine dimers we provide direct evidence for the presence ultraviolet-induced damage purified from any nuclear contamination. Previous studies been limited, they focused on frequency single deletion. Therefore addressed question spectrum skin whether can used an index overall damage. long polymerase chain reaction technique to determine deletion almost entire genome 71 split samples relation sun exposure. There was significant increase number with increasing epidermis (Kruskal-Wallis test, p = 0.0015) but not dermis (p 0.6376). The findings are confounded by age-dependent increases also detected technique. large identified our highlights ubiquitous nature high mutational load associated chronologic aging. Compared detection competitive reaction, show is sensitive therefore more comprehensive, although quantitative,