Quantitative Analysis of AML1-ETO Fusion Transcripts in t(8;21) Positive AML Using Real-Time RT-PCR

摘要: Acute myeloid leukemia (AML) is an aggressive hematologic malignancy that requires urgent cytostatic treatment. The incidence of AML approximately 2–4 new cases per 100,000 with a median age about 65 years. Despite intensive treatment cytotoxic drugs and bone marrow transplantation, long-term survival less than 30% [1, 2]. In early progenitor transformed to leukemic blast proliferates in blood suppresses normal hematopoiesis. 55% the blasts show cytogenetic abnormalities may be associated distinct biological clinical features. These specific can detected via laboratory techniques like classical cytogenetics, FISH, Southern blotting or PCR. karyotype clone not only one most important prognostic factors AML, but it also facilitates monitoring minimal residual disease at times cytomorphologic remission when undetectable by light microscopy. At primary diagnosis estimated burden 1012 malignant cells. Complete after chemotherapy defined as return cytomorphology 5% blasts. However, state patients still have 1010 relapse common cause failure [4]. kinetics regrowth host immune response influence remain largely unknown.