Acetyl-l-carnitine partially prevents benzene-induced hematotoxicity and oxidative stress in C3H/He mice.

摘要: Benzene is an environmental pollutant and occupational toxicant which induces hematotoxicity. Our previous metabonomics study suggested that acetyl-l-carnitine (ALCAR) decreased in the mouse plasma bone marrow (BM) cells due to benzene exposure. In present study, topic on whether ALCAR influences hematotoxicity caused by exposure was explored. Thirty-two male C3H/He mice were divided into four groups: control group (C: vehicle, oil), (150mg/kg body weight (b.w.) benzene), benzene+A1 b.w. benzene+100mg/kg ALCAR), benzene+A2 benzene+200mg/kg ALCAR). injected subcutaneously, orally administrated via gavage once daily for 4 weeks consecutively. After experimental period, blood routine, BM cell number frequency of hematopoietic stem/progenitor (HS/PC) assessed. The mitochondrial membrane potential ATP level determined evaluate function. Reactive oxygen species (ROS), hydrogen peroxide (H2O2) malondialdehyde (MDA) levels also examined, comet assay performed measure oxidative stress. Results showed intervention can partially reduce benzene-induced damage HS/PCs simultaneously alleviate DNA reducing H2O2, ROS, MDA.