Ultrasensitive reporter protein detection in genetically engineered bacteria

摘要: We demonstrate the use of laser-induced fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates and, since spectroscopic focal volume approximately size one cell, also individual live bacteria. This is, our knowledge, first ever proof-of-concept work utilizing instrumentation with single-molecule detection capability monitor response. Although we arsenic inducible bioreporters here, method extensible gfp/egfp that are responsive other substances.