作者: Åke Larsson , Herbert F. Helander
DOI: 10.1007/BF02060286
关键词: Dentinogenesis 、 Inorganic chemistry 、 Pyrophosphate 、 Inorganic pyrophosphatase 、 Staining 、 Stratum intermedium 、 Chemistry 、 Electron microscope 、 Dentin 、 Mineralization (biology)
摘要: After incubation of aldehyde-fixed sections rat molar germs in an alkaline solution lead pyrophosphate, a positive sulphide staining reaction was observed the mineralization front dentin, stratum intermedium, and subodontoblastic cells. In electron microscope, number size visible needle-like crystallites increased zone (intermediate dentin). A fine precipitate predominantly nuclei intermedium The effects EDTA, Zn2+, Ca2+, Mg2+ citrate were also studied probability inorganic pyrophosphatase activities is discussed relation to deposits. interaction cannot be excluded precipitation phosphate. However, may due uncatalyzed adsorption compound onto apatite crystals.