Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS.

摘要: During bloodstream infections, rapid adaptation of empirical treatment according to the microorganism identified is essential decrease mortality. The aim present study was assess microbiological performances a new version Sepsityper® kit (Bruker Daltonics) allowing identification bacteria and yeast by MALDI-TOF mass spectrometry directly from positive blood cultures in 10 min specific MBT-Sepsityper module for spectra analysis, designed increase performance. Identification rates were determined prospectively on 350 bacterial 29 fungal cultures, compared conventional diagnostic method. Our diagnosis strategy (Rapid protocol: one spot with without formic acid extraction step) combined provided 65.4%, 78.9% 62% reliable species level monomicrobial growing respectively Gram-positive, Gram-negative or yeast. Importantly, Gram-positive higher anaerobic than aerobic bottles (77.8% vs 22.2%; p = 0.004), if step performed (60.8% 39.2%; p = 1.8e−6) used (76.2% 61.9%, p = 0.041) while no significant differences observed bacteria. For yeasts identification, improved rate 37.9% increased overall 38%, providing up 89.7% associated standard protocol. These performances, reduce turnaround time, may help implement infections routine workflow microbiology laboratories.