Live-Cell Dynamics and the Role of Costimulation in Immunological Synapse Formation
作者： Scott A. Wetzel , Timothy W. McKeithan , David C. Parker
DOI: 10.4049/JIMMUNOL.169.11.6092
关键词: T-cell receptor 、 Immunological synapse formation 、 CD80 、 Immunological synapse 、 CD28 、 Synapse 、 T cell 、 Cell biology 、 Biology 、 Video microscopy
摘要: Using transfected fibroblasts expressing both wild-type I-E k and green fluorescent protein-tagged with covalently attached antigenic peptide, we have monitored movement of specific MHC:peptide complexes during CD4 + T cell-APC interactions by live-cell video microscopy. Ag recognition occurs within 30 s contact, as shown a sharp increase in cytoplasmic calcium ion concentration. Within 1 min, small clusters form the contact zone that coalesce into an immunological synapse over 3–20 min. When cells conjugated to APC move across surface, they appear drag them. This system was used examine role costimulation formation synapse. Blocking CD80/CD28 or ICAM-1/LFA-1 alters morphology reduces area density accumulated complexes. These reductions correlate reduced cell proliferation, while CD69 CD25 expression TCR down-modulation remain unaffected. Thus, is essential for normal mature formation.
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