Pubertal mouse mammary gland development - transcriptome analysis and the investigation of Fbln2 expression and function

摘要: Mouse mammary gland morphogenesis at puberty is a complex developmental process, regulated by systemic hormones, local growth factors and dependent on the epithelial/epithelial epithelial/stromal interactions. TEBs which invade fat pad are important in laying down epithelial framework of this time point. The objective thesis was to use combination ‘pathway-‘ ‘candidate gene analysis’ transcriptome isolated ducts associated stroma, combined with detailed analyses selected proteins, further define key proteins processes involved puberty. Using GeneChip® Exon 1.0 ST Arrays we identified epithelial-, epithelial-stromal- stromal transcriptomes defined major functional pathways/biological each compartment. By ranking transcripts according their expression levels known functions other systems, genes potential importance for pubertal morphogenesis. We focused our study Upk3a Fbln2 protein products. could only be detected mRNA level thus analysis based Fbln2. demonstrated that V1 predominantly expressed epithelium stroma TEBs. hormone primed mice localisation mouse positively E2 P. Furthermore, silico analysis, vitro assays, IHC IF Vcan, Lama1, Fbn1, ColVIαIII, ColIVαI, ColXVIIIαI, Eln, Per, Acan, Nid, Itgb3 Itga5 as binding partners gland. Finally, reported lack an obvious phenotype KO-/- but may attributed over-compensation Fbln1. This demonstrates benefit DNA microarray studying development It identifies regulator interacting various ECM different sites milieu contribute array structural migratory during These data substantially add understanding reveal many avenues investigations.