The effect of pulsed electric fields on biological cells: experiments and applications
作者： K.H. Schoenbach , F.E. Peterkin , R.W. Alden , S.J. Beebe
DOI: 10.1109/27.602501
关键词: Field (physics) 、 Optoelectronics 、 Biofouling 、 Electric field 、 Water cooling 、 Materials science 、 Pulse duration 、 Range (particle radiation) 、 Electric potential energy 、 Coolant 、 Electrical engineering
摘要: The effect of pulsed electric fields with amplitudes in the range 100 V/cm-100 kV/cm on bacteria and aquatic nuisance species has been explored. pulse duration was so short that heating biological matter could be neglected. electrical energy required for lysing bacteria, or stunning species, decreases when is reduced. For Eschericia coli, this tendency proven to hold pulsewidths as 60 ns. macroorganisms, however, it found less than 5 /spl mu/s, reversed: affect macroorganisms increases again. This minimum energy, maximum efficiency, respectively, can understood by taking time charging cell membrane into account. Applications field technique (PEFT) are biofouling prevention, debacterialization liquids, medicine. A series tests prevention a cooling system untreated water coolant demonstrated economic feasibility electro-technology.
harvard.edu
sci-hub.se 参考文章(14)
A.J.H. Sale, W.A. Hamilton, Effects of high electric fields on micro-organisms. 3. Lysis of erythrocytes and protoplasts. Biochimica et Biophysica Acta. ,vol. 163, pp. 37- 43 ,(1968) , 10.1016/0005-2736(68)90030-8
Claudio A. Nicolini, Herman P. Schwan, A. Chiabrera, Interactions between electromagnetic fields and cells Published in cooperation with NATO Scientific Affairs Division [by] Plenum Press. ,vol. 97, ,(1985)
Jay S. Pearlman, Joseph E. Dunn, Andrew H. Bushnell, R. Wayne Clark, High pulsed voltage systems for extending the shelf life of pumpable food products ,(1990)
K.H. Schoenbach, F.E. Peterkin, S. Beebe, D. Byars, R. Alden, P. Adolphson, T. Turner, Effect of pulsed electric fields on micro-organisms: experiments and applications ieee international pulsed power conference. ,vol. 1, pp. 25- 33 ,(1995) , 10.1109/PPC.1995.596450
D.C. Chang, T.S. Reese, Changes in membrane structure induced by electroporation as revealed by rapid-freezing electron microscopy Biophysical Journal. ,vol. 58, pp. 1- 12 ,(1990) , 10.1016/S0006-3495(90)82348-1
A. L. Hodgkin, A. F. Huxley, A quantitative description of membrane current and its application to conduction and excitation in nerve The Journal of Physiology. ,vol. 117, pp. 500- 544 ,(1952) , 10.1113/JPHYSIOL.1952.SP004764
Gunter A. Hofmann, Glen A. Evans, Electronic Genetic-Physical and Biological Aspects of Cellular Electromanipulation IEEE Engineering in Medicine and Biology Magazine. ,vol. 5, pp. 6- 25 ,(1986) , 10.1109/MEMB.1986.5006335
H. H�lsheger, J. Potel, E. -G. Niemann, Killing of bacteria with electric pulses of high field strength. Radiation and Environmental Biophysics. ,vol. 20, pp. 53- 65 ,(1981) , 10.1007/BF01323926
Herman P. Schwan, Electric Characteristics of Tissues Radiation and Environmental Biophysics. ,vol. 1, pp. 198- 208 ,(1963) , 10.1007/BF01195396
K. S. Cole, Electric impedance of marine egg membranes Transactions of The Faraday Society. ,vol. 33, pp. 966- 972 ,(1937) , 10.1039/TF9373300966