Flagellar and ciliary beating in trypanosome motility.

摘要: The single flagellum of Leishmania and Trypanosoma parasites is becoming an increasingly attractive model for the analysis flagellar function—driven largely by abundance genomic proteomic information available organelle, genetic manipulability organisms importance motility parasite lifecycle. However, as yet, there a paucity published data on beating any genetically malleable trypanosomatid species. Here we undertook in-depth using high-speed videomicroscopy free-swimming major cells in comparison to Crithidia species (for which some existing literature). In so doing, describe simple generally-applicable technique facilitate quantitative cells. Our thoroughly defines parameters expected tip-tobase symmetrical beat these It also describes initiation from points other than tip completely different, base-to-tip highly-asymmetric that represents ciliary flagella. Moreover, detailed parameter interrelationships revealed unexpected dependency wavelength oscillator length may be result reversible constraint doublet sliding at or resonance beat. Cell Motil. Cytoskeleton 64:629–643, 2007. ' 2007 Wiley-Liss, Inc.