An avirulent ICP34.5 deletion mutant of herpes simplex virus type 1 is capable of in vivo spontaneous reactivation.

摘要: The herpes simplex virus type 1 (HSV-1) ICP34.5 gene is a neurovirulence in mice. In addition, some mutants have been reported to reduced efficiency of induced reactivation as measured by vitro explantation latently infected mouse ganglia. However, since spontaneous almost nonexistent mice, nothing has on the effect vivo. To examine this, we deleted both copies from strain HSV-1 (McKrae) that high rate rabbits and used this mutant infect rabbit eyes. All with (d34.5) survived, even at challenge doses greater than 4 x 10(7) PFU per eye. contrast, 200-fold-lower dose 2 10(5) eye was lethal for approximately 50% either wild-type McKrae parental or rescued which were restored. over 10(6) PFU, compared value less 10 virus. restricted replication eyes trigeminal ganglia 1.4% determined culturing tear films presence reactivated This more 10-fold lower (19.6%) highly significant (P < 0.0001, Fisher exact test). Southern analysis confirmed retained deletion. Thus, report demonstrates (i) gene, known be also important virulence (ii) vivo ocular model, although reduced, can occur absence gene.