Functional intratumoral lymphatics in patient-derived xenograft models of squamous cell carcinoma of the uterine cervix: implications for lymph node metastasis
作者： Einar K. Rofstad , Ruixia Huang , Kanthi Galappathi , Lise Mari K. Andersen , Catherine S. Wegner
DOI: 10.18632/ONCOTARGET.10931
关键词: Immunohistochemistry 、 Metastasis 、 Medicine 、 Lymphangiogenesis 、 Carcinoma 、 Pathology 、 Angiogenesis 、 Lymph node 、 Lymph 、 Lymphatic system 、 Oncology
摘要: Studies of cell line-derived human tumor xenografts have suggested that the lymphatics seen in immunohistochemical preparations from non-peripheral regions tumors are nonfunctional. In this investigation, lymphangiogenesis, hemangiogenesis, and lymph node metastasis were studied patient-derived xenograft (PDX) models carcinoma uterine cervix. Lymph vessel density (LVD) blood (BVD) measured preparations. The expression angiogenesis-related genes was investigated by quantitative PCR. Lymphatic functionality assessed with ferritin assay, interstitial fluid pressure (IFP) a Millar catheter. PDX mirrored angiogenesis aggressiveness donor patients' tumors, two highly aggressive developed functional within mass. Tumors intratumoral showed low IFP, high LVD, BVD, large number genes, incidence metastases. LVD correlated associated BVD. Nine development lymhatics identified. High these BVD may be important biomarkers for poor outcome cervix carcinoma.
impactjournals.com参考文章(44)
J. P. Sleeman, The Lymph Node as a Bridgehead in the Metastatic Dissemination of Tumors Recent Results in Cancer Research. ,vol. 157, pp. 55- 81 ,(2000) , 10.1007/978-3-642-57151-0_6
Diane R. Bielenberg, Bruce R. Zetter, The Contribution of Angiogenesis to the Process of Metastasis The Cancer Journal. ,vol. 21, pp. 267- 273 ,(2015) , 10.1097/PPO.0000000000000138
N Weidner, Intratumor microvessel density as a prognostic factor in cancer. American Journal of Pathology. ,vol. 147, pp. 9- 19 ,(1995)
Christopher D. Willey, Ashley N. Gilbert, Joshua C. Anderson, George Yancey Gillespie, Patient-Derived Xenografts as a Model System for Radiation Research Seminars in Radiation Oncology. ,vol. 25, pp. 273- 280 ,(2015) , 10.1016/J.SEMRADONC.2015.05.008
Einar K. Rofstad, Kanthi Galappathi, Berit S. Mathiesen, Tumor Interstitial Fluid Pressure—A Link between Tumor Hypoxia, Microvascular Density, and Lymph Node Metastasis Neoplasia. ,vol. 16, pp. 586- 594 ,(2014) , 10.1016/J.NEO.2014.07.003
Louis M. Sherwood, Edith E. Parris, Judah Folkman, Tumor Angiogenesis: Therapeutic Implications New England Journal of Medicine. ,vol. 285, pp. 1182- 1186 ,(1971) , 10.1056/NEJM197111182852108
P.B. Vermeulen, G. Gasparini, S.B. Fox, C. Colpaert, L.P. Marson, M. Gion, J.A.M. Beliën, R.M.W. de Waal, E. Van Marck, E. Magnani, N. Weidner, A.L. Harris, L.Y. Dirix, Second international consensus on the methodology and criteria of evaluation of angiogenesis quantification in solid human tumours. European Journal of Cancer. ,vol. 38, pp. 1564- 1579 ,(2002) , 10.1016/S0959-8049(02)00094-1
Sarah Jane Lunt, Tuula MK Kalliomaki, Allison Brown, Victor X Yang, Michael Milosevic, Richard P Hill, Interstitial fluid pressure, vascularity and metastasis in ectopic, orthotopic and spontaneous tumours BMC Cancer. ,vol. 8, pp. 2- 2 ,(2008) , 10.1186/1471-2407-8-2
Rui-Cheng Ji, Lymphatic endothelial cells, tumor lymphangiogenesis and metastasis: New insights into intratumoral and peritumoral lymphatics. Cancer and Metastasis Reviews. ,vol. 25, pp. 677- 694 ,(2007) , 10.1007/S10555-006-9026-Y
S.D. Nathanson, R. Shah, K. Rosso, Sentinel lymph node metastases in cancer: causes, detection and their role in disease progression. Seminars in Cell & Developmental Biology. ,vol. 38, pp. 106- 116 ,(2015) , 10.1016/J.SEMCDB.2014.10.002