Transcriptome analysis of tube foot and large scale marker discovery in sea cucumber, Apostichopus japonicus
作者： Xiaoxu Zhou , Hongdi Wang , Jun Cui , Xuemei Qiu , Yaqing Chang
DOI: 10.1016/J.CBD.2016.07.005
关键词: Sea cucumber 、 Gene 、 Transcriptome 、 Apostichopus japonicus 、 Gene expression profiling 、 Biology 、 Genome 、 Genetics 、 Botany 、 Appendage 、 Function (biology)
摘要: Abstract Tube foot as one of the ambulacral appendages types in Aspidochirote holothurioids, is known for their functions locomotion, feeding, chemoreception, light sensitivity and respiration. In this study, we explored characteristic transcriptome tube sea cucumber (Apostichopus japonicus). Our results showed that among 390 unigenes which specifically expressed foot, 190 them were annotated. Based on assembly transcriptome, found 219,860 SNPs from 34,749 unigenes, 97,683, 53,624, 27,767 40,786 located CDSs, 5′-UTRs, 3′-UTRs non-CDS separately. Furthermore, 12,114 SSRs detected 7394 unigenes. Target genes four miRNAs (miR-29a, miR-29b, miR-278-3p miR-2005) also predicted based contain immune-related factors (MBL, VLRA, AjC3, MyD88, CFB), skin pigmentation (MITF), candidate regeneration factor (TRP) holothurians autolysis-related (CL). These develop a relatively large number molecular markers resources, will provide foundation further analyses function mechanisms underlying A. japonicas foot.
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