Interaction of SiFe Nanoparticles with Epithelial and Lymphoid Cells

摘要: Silicon and silicon-based nanoparticles (SiNP) attract scientific attention due to the biocompatibility assimilation of silicon by body tissues. Iron-doped SiNP (SiFeNP) allow use ferromagnetic properties iron for NP detection possibility therapeutic application SiFeNP. The purpose this work was analyze interaction SiFeNP with epithelial cells (EC) COLO357 SW620 human peripheral blood lymphocytes (PBL). were obtained laser ablation divided into NP1 NP2 fractions 100 150 nm size, respectively. Cytotoxicity, apoptosis induction, reactive oxygen species (ROS) production, lysosome metabolism analyzed using in vitro methods. EC found efficiently incytosed both types NPs, which resulted increase granularity cells. did not cause or necrosis, but accumulated lysosomes, led a decrease membrane potential lysosomes. In turn, level gradual (24 h) ROS production 10–15%. caused more than NP2, EC, may be result difference particle size. interact PBL. Thus, total cytotoxicity exceed 20%, is associated insignificant production.