Establishment of immortalized mesenchymal stem cells derived from the submandibular glands of tdTomato transgenic mice.

摘要: Transgenic mice that overexpress the red fluores - cent protein tdTomato (tdTomato mice) are well suited for use in regenerative medicine studies. Cultured cells from this murine model exhibit strong fluorescence, enabling real-time vivo imaging through body surface of grafted animals. Mesenchymal stem (MSCs) have marked potential cell therapy and medicine; however, mechanisms regulate their dynamics poorly understood. In present study, an MSC line was derived submandibular gland fibroblasts mice. The fluorescent signal observed organs throughout body, as salivary glands. Primary culture were immortalized with SV40 large T antigen (GManSV cells); these exhibited increased migratory ability, compared those isolated sublingual gland. GManSV tdTomato‑positive spindle‑shaped fibroblastic morphology; they also robustly expressed mouse markers: Stem antigen‑1 (Sca‑1), CD44, CD90. This retained multipotent characteristics, evidenced by its ability to differ- entiate into both osteogenic adipogenic lineages. These results indicate Sca‑1 + /CD44 /CD90 ‑GManSV may be useful kinetic studies gland‑derived MSCs context vitro co‑culture other types cells. used studies, order identify novel treatment diseases.