Cathepsins B and L Increased during Response of Periodontal Ligament Cells to Mechanical Stress In Vitro

摘要: Cathepsin is a typical and well-characterized lysosomal cysteine protease that, under pathological conditions, involved in tissue destruction. A recent immunocytochemical study demonstrated that cathepsins B (CAB) L (CAL) were localized the periodontal ligament (PDL) of rat molar, they expressed compressed sites during experimental tooth movement. Further, we previously levels CAB CAL gingival crevicular fluid increased orthodontic Therefore, may play important roles process collagen degradation movement, our vitro examined secretion PDL cells following mechanical stress. subjected to 0.5, 1.0, 2.0, or 3.0 g/cm2 compression force an increase surface area by tension 0.28%, 0.95%, 1.72%, 2.50% for 24 hr. For detection conditioned medium, commercially available ELISA kits used. We found significantly secretions both cells, which exhibited time- magnitude-dependent manner. The compression-stimulated was approximately 3-fold 4-fold, as compared with control. tension-stimulated 1.5-fold 2-fold When analyzed using semiquantitative polymerase chain reaction assay, mRNA response forces. These findings stress (compression forces) causes vitro.