Proteomic changes after fertilization and before first cleavage in rainbow trout Oncorhynchus mykiss

摘要: Abstract In most vertebrates, the development of a new individual starts with fertilization oocyte by spermatozoon. A label-free quantification strategy was applied to investigate proteomic changes after and before first cleavage in egg rainbow trout. Samples were obtained from fertilized eggs at 50, 200 min, 15 h 4 °C. Significant observed abundances thirty-two proteins different time points. The accompanied an upregulation coagulation factor protein which might be responsible for construction layer protection developing embryo. Upregulation iron binding such as ferritin ceruloplasmin could reflect importance this metal ion fertilization. quantity complement is known immunological significantly increased during early stages. kinase potentials phosphorylation. Importin vitellogenin showed both down- patterns. timing three types actins may indicate their potential roles egg. Kinesin-like protein, Sororin etc. contribute cell preparation division upcoming cleavage. conclusion, insemination causes extensive have conduct Data are available via ProteomeXchange identifier PXD013329 .