Chromosome in situ hybridization on formalin-fixed mammary tissue using non-isotopic, non-fluorescent probes: technical considerations and biological implications.

摘要: Fluorescentin situ hybridization techniques have provided an important tool for interphase cytogenetic studies of human neoplasms. However, these are difficult to use on formalin-fixed archival tissue sections. We describe here a non-fluorescent, non-isotopicin (ISH) approach that is easily applicable paraffin-embedded breast The technical steps must be monitored and individualized optimize signal generation detection discussed. This ISH technique has several advantages over fluorescent methods. obtained can viewed using ordinary light microscope does not fade with time. More importantly, the observed analyzed in context morphology. permits numerical chromosomal abnormalities only malignant but also apparently normal potentially premalignant mammary tissue. may allow identification focal genetic as well field-defects enable analysis their evolution during multistep transformation neoplasm. suitable tumor heterogeneity correlation aberrations histologic, immunocytochemical, clinical features tumors.