Hypergravity-induced immunomodulation in a rodent model: lymphocytes and lymphoid organs.

摘要: The major goal of this study was to quantify changes in lymphoid organs and cells over time due centrifugation-induced hypergravity. C57BL/6 mice were exposed 1, 2 3 G the following assays performed on days 4, 7, 10, 21: spleen, thymus, lung, liver masses; total leukocyte, lymphocyte, monocyte/macrophage, granulocyte counts; level splenocyte apoptosis; enumeration CD3+ T, CD3+/CD4+ T helper, CD3+/CD8+ cytotoxic, B220+ B, NK1.1+ natural killer cells; quantification expressing CD25, CD69, CD71 activation markers. data show that increased gravity resulted decreased body, liver, but not mass. Significant reductions noted all three leukocyte populations (lymphocytes, granulocytes, monocyte/macrophages) [correction macrphages] with gravity; persistent depletion blood spleen. Among various lymphocyte populations, B most affected NK least affected. Overall, evident during first week, a greater influence for A linear relationship found between some measurements gravity, especially day 4. These findings indicate hypergravity profoundly alters number distribution mammalian model aberrations persisted throughout weeks study. In certain cases, detected similar those observed after whole-body irradiation. future investigations we hope combine low-dose rate irradiation immune challenge.