Purification and Physical Properties of E. coli RNA Polymerase

摘要: INTRODUCTION The purpose of this review is to present a summary (1) the variables involved in purification E. coli DNA-dependent RNA polymerase (E.C. 2.7.7.6), (2) criteria by which purity enzyme can be assessed, and (3) physical properties its subunits. It hoped that will provide useful guide choosing method reference for enzyme. No attempt made all-inclusive or historical, but rather most recent thorough studies emphasized. Several reviews covering similar material have appeared (Richardson 1969; Geiduschek Haselkorn Burgess 1971; Chamberlin 1974a, b). This deal with holoenzyme, core sigma factor. Holoenzyme has subunit composition α 2 ββ ′ σ resolved into two components: ( ′) factor ). Most researchers studied mixture holoenzyme polymerase. appears synthesis cellular RNA, possible exception certain primers DNA replication, as discussed Kornberg (this volume). A variety other forms been reported some cases, named: II found stationary cells (Chao Speyer 1973); small amounts consisting polypeptide termed (Fukuda, Iwakura Ishihama 1974); series activities crude extracts different sedimentation template (Snyder...