Role of gene amplification in drug resistance

摘要: The drug-resistant phenotype is prevalent in many human cancers. In particular, multidrug resistance, which cells are simultaneously resistant to several classes of anticancer agents, poses a major obstacle the treatment cancer. One strategy elucidate drug resistance mechanisms operative cancer independently derive cell lines vitro that mimic patterns observed vivo. Drug-resistant mammalian selected may have highly amplified genomic sequences encode large amounts target proteins or detoxifying systems for variety cytotoxic agents [1,2]. These particularly useful genetic, biochemical, and molecular analysis because they provide excellent starting materials cloning respective genes.