Fluorouracil induces autophagy-related gastric carcinoma cell death through Beclin-1 upregulation by miR-30 suppression
作者： Chun Yang , Yong Pan
DOI: 10.1007/S13277-015-3775-6
关键词: Translation (biology) 、 Cell growth 、 Cell counting 、 Downregulation and upregulation 、 Programmed cell death 、 Molecular biology 、 Autophagy 、 microRNA 、 Fluorouracil 、 Biology
摘要: The molecular mechanisms underlying the anti-cancer effects of chemotherapy drugs are not completely understood. Here, we studied fluorouracil (5-FU) on gastric carcinoma (GC) cells. We found that 5-FU dose-dependently inhibited growth GC cells, in either a cell counting kit-8 (CCK-8) assay or 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, autophagy-associated protein 6 (ATG6) Beclin-1 was activated by Further, microRNA (miR)-30 to be regulated 5-FU, and bioinformatics analysis showed miR-30 targeted 3′-UTR inhibit its translation. Together, these data suggest may suppress upregulate induce autophagic death proliferation arrest
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Weidong Wu, Honghua Ding, Jun Cao, Weihao Zhang, None, FBXL5 Inhibits Metastasis of Gastric Cancer Through Suppressing Snail1 Cellular Physiology and Biochemistry. ,vol. 35, pp. 1764- 1772 ,(2015) , 10.1159/000373988
Ying Wang, Shegan Gao, Gang Liu, Ruinuo Jia, Daiming Fan, Xiaoshan Feng, Microarray expression profile analysis of long non-coding RNAs in human gastric cardiac adenocarcinoma. Cellular Physiology and Biochemistry. ,vol. 33, pp. 1225- 1238 ,(2014) , 10.1159/000358692
I. Martinez, D. Cazalla, L. L. Almstead, J. A. Steitz, D. DiMaio, miR-29 and miR-30 regulate B-Myb expression during cellular senescence Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 522- 527 ,(2011) , 10.1073/PNAS.1017346108
Yingjun Cui, Jie Chen, Zhixian He, Yongtao Xiao, SUZ12 depletion suppresses the proliferation of gastric cancer cells. Cellular Physiology and Biochemistry. ,vol. 31, pp. 778- 784 ,(2013) , 10.1159/000350095
Samir Attoub, Sherif M. Karam, Abderrahim Nemmar, Kholoud Arafat, Annie John, Wafa Al-Dhaheri, Mahmood Ahmed Al Sultan, Haider Raza, Short-term effects of oral administration of Pistacia lentiscus oil on tissue-specific toxicity and drug metabolizing enzymes in mice. Cellular Physiology and Biochemistry. ,vol. 33, pp. 1400- 1410 ,(2014) , 10.1159/000358706
F Yu, H Deng, H Yao, Q Liu, F Su, E Song, Mir-30 reduction maintains self-renewal and inhibits apoptosis in breast tumor-initiating cells Oncogene. ,vol. 29, pp. 4194- 4204 ,(2010) , 10.1038/ONC.2010.167
Fei Wang, Weizhong Xiao, Jiyong Sun, Donghua Han, Youhou Zhu, MiRNA-181c inhibits EGFR-signaling-dependent MMP9 activation via suppressing Akt phosphorylation in glioblastoma Tumor Biology. ,vol. 35, pp. 8653- 8658 ,(2014) , 10.1007/S13277-014-2131-6
Gianpiero Di Leva, Carlo M Croce, miRNA profiling of cancer Current Opinion in Genetics & Development. ,vol. 23, pp. 3- 11 ,(2013) , 10.1016/J.GDE.2013.01.004
Jihong Zhang, Marc Hummersone, Charles S. Matthews, Malcolm F.G. Stevens, Tracey D. Bradshaw, N3-substituted temozolomide analogs overcome methylguanine-DNA methyltransferase and mismatch repair precipitating apoptotic and autophagic cancer cell death. Oncology. ,vol. 88, pp. 28- 48 ,(2015) , 10.1159/000366131
C-J Kao, A Martiniez, X-B Shi, J Yang, C P Evans, A Dobi, R W deVere White, H-J Kung, miR-30 as a tumor suppressor connects EGF/Src signal to ERG and EMT. Oncogene. ,vol. 33, pp. 2495- 2503 ,(2014) , 10.1038/ONC.2013.200