Identification and characterization of the human mus81-eme1 endonuclease.
作者： Alberto Ciccia , Angelos Constantinou , Stephen C. West
关键词: Endonuclease complex 、 Origin recognition complex 、 Ter protein 、 Pre-replication complex 、 Minichromosome maintenance 、 Biology 、 Control of chromosome duplication 、 Flap structure-specific endonuclease 1 、 Genetics 、 Replication factor C
摘要: Abstract The faithful and complete replication of DNA is necessary for the maintenance genome stability. It known, however, that forks stall at lesions in template need to be processed so restart can occur. In fission yeast, Mus81-Eme1 endonuclease complex (Mus81-Mms4 Saccharomyces cerevisiae) has been implicated processing aberrant intermediates. this report, we identify human homolog Schizosaccharomyces pombe EME1 gene have purified heterodimer. We show an exhibits a high specificity synthetic fork structures 3′-flaps vitro. nuclease cleaves Holliday junctions inefficiently (∼75-fold less than flap or structures), although cleavage increased 6-fold by presence homologous sequences previously shown permit base pair “breathing.” conclude flap/fork likely play role stalled
sci-hub.se 参考文章(36)
F.E. Benson, S.C. West, Substrate specificity of the Escherichia coli RuvC protein. Resolution of three- and four-stranded recombination intermediates. Journal of Biological Chemistry. ,vol. 269, pp. 5195- 5201 ,(1994) , 10.1016/S0021-9258(17)37674-3
P Sung, P Reynolds, L Prakash, S Prakash, Purification and characterization of the Saccharomyces cerevisiae RAD1/RAD10 endonuclease. Journal of Biological Chemistry. ,vol. 268, pp. 26391- 26399 ,(1993) , 10.1016/S0021-9258(19)74327-0
Kyungjae Myung, Abhijit Datta, Clark Chen, Richard D. Kolodner, SGS1, the Saccharomyces cerevisiae homologue of BLM and WRN, suppresses genome instability and homeologous recombination. Nature Genetics. ,vol. 27, pp. 113- 116 ,(2001) , 10.1038/83673
Paul M. Watt, Edward J. Louis, Rhona H. Borts, Ian D. Hickson, SGS1, a Homologue of the Bloom's and Werner's Syndrome Genes, Is Required for Maintenance of Genome Stability in Saccharomyces cerevisiae Genetics. ,vol. 144, pp. 935- 945 ,(1996) , 10.1093/GENETICS/144.3.935
Peter McGlynn, Robert G. Lloyd, Recombinational repair and restart of damaged replication forks Nature Reviews Molecular Cell Biology. ,vol. 3, pp. 859- 870 ,(2002) , 10.1038/NRM951
Angelos Constantinou, Adelina A Davies, Stephen C West, Branch Migration and Holliday Junction Resolution Catalyzed by Activities from Mammalian Cells Cell. ,vol. 104, pp. 259- 268 ,(2001) , 10.1016/S0092-8674(01)00210-0
Elspeth Stewart, Carolyn Riley Chapman, Fahad Al‐Khodairy, Antony M Carr, Tamar Enoch, rqh1+, a fission yeast gene related to the Bloom‘s and Werner's syndrome genes, is required for reversible S phase arrest The EMBO Journal. ,vol. 16, pp. 2682- 2692 ,(1997) , 10.1093/EMBOJ/16.10.2682
Matthew C. Whitby, Fekret Osman, Julie Dixon, Cleavage of Model Replication Forks by Fission Yeast Mus81-Eme1 and Budding Yeast Mus81-Mms4 Journal of Biological Chemistry. ,vol. 278, pp. 6928- 6935 ,(2003) , 10.1074/JBC.M210006200
José Antonio Tercero, John F. X. Diffley, Regulation of DNA replication fork progression through damaged DNA by the Mec1/Rad53 checkpoint Nature. ,vol. 412, pp. 553- 557 ,(2001) , 10.1038/35087607
Jennifer A Cobb, Lotte Bjergbaek, Susan M Gasser, RecQ helicases: at the heart of genetic stability. FEBS Letters. ,vol. 529, pp. 43- 48 ,(2002) , 10.1016/S0014-5793(02)03269-6