New approaches to preparation and storage of platelets

摘要: During the last century, transfusion of blood components has become a prerequisite for treatment in many clinical settings. The availability plastic bags and methods centrifugation separation cells, enabled processing whole into cell components, made it possible to optimize therapy, as well storage conditions. Automation processes been introduced, which increases reproducibility standardization prepared components. Recommendations leukocyte reduction minimize side-effects caused by concomitant leukocytes have resulted development different types filters. Today, universal is practice countries Sweden, majority are reduced. For immunosuppressed patients, who at risk developing associated graft-versus host disease attributed passenger T-lymphocytes, there need further restrain proliferation Tlymphocytes, hence prevent TA-GVHD. prevalent method gamma irradiation, recent years X-ray irradiation available another alternative from radioactive sources. studies included present thesis focus on 1) preparation techniques saves platelet, results erythrocytes, plasma platelets, 2) automated reduced platelets pooled buffy coats platelet content selection coats, 3) evaluation additive solutions with composition, aim conditions 4) effects gammaand during storage. indicate that filter sufficiently low leukocytes, satisfactory recovery erythrocytes high quality measured vitro parameters, were maintained Automated OrbiSac was introduced. Prepared displayed characteristics equivalent previous manual method, 7 days. Moreover, count variation Evaluation concluded addition phosphate beneficial platelets. This finding had an impact next generations solutions. Effects equipments studied. No major deleterious identified. data scarce, especially our imply does not compromise all represent new options component preparation, enhanced efficacy safety. LIST OF PUBLICATIONS I Larsson S, Gulliksson H, Paunovic D. whole-blood WBCreduction platelets: studies. Transfusion 2001;41:534-539. II Sandgren P, Sjodin A, Vesterinen M, H. concentrates coats: experiences system. 2005;45:743-751. III Kumlien G, Shanwell A. Storage Platelets Additive Solutions: Phosphate. Vox Sang 2000;78:176-184. IV Kallman A-S, Lundahl J, Irradiation two techniques: cesium X-rays. Submitted (Transfusion).