Lucky Imaging: Improved Localization Accuracy for Single Molecule Imaging
作者： Bríd Cronin , Ben de Wet , Mark I. Wallace
DOI: 10.1016/J.BPJ.2008.12.3945
关键词: Preclinical imaging 、 Nuclear magnetic resonance 、 Lucky imaging 、 Nanotechnology 、 Chemistry 、 Single-molecule experiment 、 Fluorescence 、 Single Molecule Imaging 、 Resolution (electron density) 、 Quantum dot 、 Microscopy
摘要: We apply the astronomical data-analysis technique, Lucky imaging, to improve resolution in single molecule fluorescence microscopy. show that by selectively discarding data points from individual single-molecule trajectories, imaging can be improved a factor of 1.6 for fluorophores and up 5.6 more complex images. The method is illustrated using images fluorescent dye molecules quantum dots, vivo fluorescently labeled linker activation T cells.
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