MicroRNA-7450 regulates non-thermal plasma-induced chicken Sertoli cell apoptosis via adenosine monophosphate-activated protein kinase activation

摘要: Non-thermal plasma treatment is an emerging innovative technique with a wide range of biological applications. This study was conducted to investigate the effect non-thermal dielectric barrier discharge on immature chicken Sertoli cell (SC) viability and regulatory role microRNA (miR)-7450. Results showed that increased SC apoptosis in time- dose-dependent manner. Plasma-induced possibly resulted from excess production reactive oxygen species via suppression antioxidant defense systems decreased cellular energy metabolism through inhibition adenosine triphosphate (ATP) release respiratory enzyme activity mitochondria. In addition, downregulated miR-7450 expression activated monophosphate-activated protein kinase α (AMPKα), which further inhibited mammalian target rapamycin (mTOR) phosphorylation SCs. A single-stranded synthetic antagomir disrupted mitochondrial membrane potential ATP level mTOR by targeting activation AMPKα, significant increases lethality. double-stranded agomir produced opposite effects these parameters ameliorated plasma-mediated apoptotic Our findings suggest involved regulation plasma-induced AMPKα signaling pathway.