Preparation of three-dimensional vascularized MSC cell sheet constructs for tissue regeneration.
作者： Liling Ren , Dongyang Ma , Bin Liu , Jinda Li , Jia Chen
DOI: 10.1155/2014/301279
关键词: Cell 、 Cell migration 、 Microvessel 、 Anatomy 、 Regeneration (biology) 、 Neovascularization 、 Cell biology 、 Mesenchymal stem cell 、 Umbilical vein 、 Chemistry 、 Tissue engineering
摘要: Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability form rich microvessel networks. In this study we engineered prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and umbilical vein endothelial as sources. hMSCs were cultured thick sheet, (HUVECs) then seeded on The single HUVEC/hMSC was folded by modified engineering technique. vitro results indicated that promoted HUVECs migration networks in horizontal vertical directions. vivo showed many blood vessels grew into after implanted subcutaneous pocket of immunodeficient mice. density higher than nonprevascularized constructs. Immunohistochemistry staining further preformed capillaries anastomosed with host vasculature functional vessels. These suggest promising potential platform wide applications tissues.
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