Metabolism of nC11 fatty acid fed to Trichoderma koningii and Penicillium janthinellum II: Production of intracellular and extracellular lipids

摘要: AbstractLittle is known about the fungal metabolism of nC10 and nC11 fatty acids their conversion into lipids. A mixed batch culture soil fungi, T. koningii P. janthinellum, was grown on undecanoic acid (UDA), a mixture UDA potato dextrose broth (UDA+PDB), PDB alone to examine metabolic during growth. We quantified seven intracellular extracellular lipid classes using Iatroscan thin-layer chromatography with flame ionization detection (TLC-FID). Gas (GC-FID) used quantify 42 individual acids. Per 150 mL culture, UDA+PDB produced highest amount (531 mg) (14.7 mg) lipids exponential phase. The content total represented 25% biomass-carbon, or 10% biomass dry weight produced. Fatty made up largest class (457 mg/150 mL culture) they were sy...