The Effects and Mechanism of miR-92a and miR-126 on Myocardial Apoptosis in Mouse Ischemia-Reperfusion Model
作者： Changhao Jiang , Ningning Ji , Gaojiang Luo , Shimao Ni , Jinbo Zong
DOI: 10.1007/S12013-014-0149-4
关键词: Western blot 、 Caspase 3 、 Evans Blue 、 TUNEL assay 、 Ischemia 、 Apoptosis 、 Biology 、 Annexin 、 Blot 、 Andrology 、 Molecular biology
摘要: Our objective was to explore the effects of miR-92a and miR-126 on myocardial apoptosis in mouse ischemia-reperfusion model further investigate underlying mechanisms. Eighteen Kunming mice were selected randomly divided into sham operation group with nine each group. Cardiac muscle tissue stained Evans blue confirm infarction ischemia. Annexin V/PI double staining used detect apoptotic rate cells, terminal-deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) number cells; Western blot expression Caspase 3 evaluate qRT-PCR myocardium, HSP70 two groups. results showed that there a large area ischemia myocardium marked infarction, suggesting successful establishment model. In group, cells mostly normal cells. flow cytometry result 5.9 % 37.0 respectively. Apoptosis detection index (AI) significantly higher than addition, (F = 32.302, P 0.000), lower 41.125, 0.000). Moreover, detected by HSP The change accordance AI However, is contrary which may be related
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