ROLE OF LAMININ POLYMERIZATION AT THE EPITHELIAL MESENCHYMAL INTERFACE IN BRONCHIAL MYOGENESIS
作者： Nand Relan , Clement Diglio , Yan Yang , Kenneth C. Palmer , Lucia Schuger
关键词: Desmin 、 Basement membrane 、 Myocyte 、 Basement membrane assembly 、 Myogenesis 、 Myosin 、 Laminin 、 Mesenchymal stem cell 、 Biology 、 Cell biology
摘要: Undifferentiated mesenchymal cells were isolated from mouse embryonic lungs and plated at subconfluent confluent densities. During the first 5 hours in culture, all negative for smooth muscle markers. After 24 that spread synthesized alpha-actin, myosin, desmin SM22 levels comparable to those of mature muscle. The did not remained SM differentiation was independent cell-cell contact or proliferation. In additional studies, undifferentiated lung cocultured with epithelial high density. aggregated into cysts surrounded by a basement membrane formed between two cell types. these cocultures, differentiated rest round Inhibition laminin polymerization an antibody globular regions beta1/gamma1 chains blocked assembly, spreading differentiation. These studies indicated have potential differentiate process is triggered their along airway membrane.
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